Grant agreement no: 286464

Project full title: Design of Novel Portable-Sensors Based  on Suspension Arrays Composed of Monoclonal Antibody, Aptamer and Bacteriophage Carrying Magnetically Loaded Nanoparticles and Surface Enhanced Raman Spectroscopy

Project acronym: NanoBacterphageSERS

Duration of the project: 48 Months

Start date of the project: 1st November 2011

List of beneficiaries:

  1. Hacettepe University (HU), Ankara, Turkey (coordinator- E.Pişkin)
  2. Biyomedtek (BMT) Biomedical Technologies-Economical Enterprise, Ankara, Turkey
  3. University of Brighton (UB), Brighton, UK
  4. Bilkent University (UNAM), Ankara, Turkey
  5. Aptares AG (APT), Berlin, Germany (recently drawn out)
  6. Gate Elektronik A.Ş. (GT), Ankara, Turkey
  7. Eliava BioPreparations Ltd. (ELV-BP), Tbilisi, Georgia

Project Abstract

This project aims to develop nanotechnology-based systems for detection of pathogenic bacteria in several aqueous media mainly in environmental water based on suspension arrays together with a portable custom-designed detection system. The six objectives (six work packages) are as follows: (i) Nanostructured sensors surfaces, in which electron-beam, photo-, soft and dip-pen lithography will be applied in the fabrication of functionalized low-cost (disposable) SERS substrates, specially designed for simultaneous Raman and Fluorescence enhancement; (ii) nanosorbents and their mixtures as suspension arrays against the target bacteria for capturing unit, in which firstly magnetically loaded nanoparticles will be produced and labeled/barcode with fluorescence dies and/or quantum dots and then bacteriophages, monoclonal antibodies, and/or aptamers will be immobilized onto these nanoparticles as bioligands to recognize and capture the target bacteria specifically; their mixtures will form the suspension arrays; (iii) target bacteria and bacteriophages; the target bacteria (as water pollutants), are Escherichia coli, Enterocccus sp., Bacteroides fragilis and Bacteroides ovatus; bacteriophages which will recognize these bacteria will be selected/produced as bioligands; (iv) aptamers; they will be selected/ modified/produced; (v)sensors/array systems; a portable “Raman Spectrometer/Fluorescence Detector System” will be designed/produced that will be used together with the nanostructured sensors platforms, and finally (vi) validation; the materials and systems developed will be validated. This is a multidisciplinary and technological project and therefore brings together experts from different disciplines both from academia and industry. Basically, the knowledge that will be developed/accumulated at lab scale during the project will be transferred to the industrial partners for prototype productions, and then these will bring back to the academicals institutions for validation tests.

Keywords: Detection; Pathogenic bacteria; Nanotechnology; Environment, Water and Food safety

Main scientific and technology objectives

Objective 1.        Design and fabrication of nanostructured sensors surfaces for SERSObjective 3.        Selection/production/characterization of target bacteria and bacteriophages

Objective 5.        Design and construction of sensors/array systems

Objective 6.        Validation of the systems developed

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